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tips and tricks for the lab column troubleshooting and

Tips and Tricks for the Lab: Column Troubleshooting

2012-8-7  A micro-column is made by using a normal, disposable, glass Pasteur pipette and is set up in much the same way as a column (Fig. 6). A solvent system that gives a lower R f value than usual should be selected, that is, an R f value of 0.2 is suitable for a micro-column, compared with that of 0.3 for a full column.

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Practical Steps in GC Troubleshooting

2017-9-7  Troubleshooting Techniques, Tips, and Tricks Mark Sinnott Application Engineer GC Columns Supplies. ... Track Events –log book-Changed column, liner, septum, syringe, etc.-Injected samples, other method, etc.-Did maintenance, cut column, inlet flush, etc. Page 5 Troubleshooting Starts with Isolating the problem ...

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HPLC Troubleshooting Guide - ResearchGate

Learn important tips and tricks for troubleshooting HPLC columns and systems. Many LC problems can be prevented with routine preventive maintenance, For example, replacing pump seals at regular ...

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Troubleshooting and Tips Tricks (G1)

2013-9-10  UPLC® column frits can plug due to bacterial growth in highly aqueous mobile phases Bacteria is the most common cause of UPLC® column plugging and can easily be avoided by filtering through a 0.22 µm filter pH 7 phosphate buffer is VERY susceptible to bacterial growth Electron micrograph of Rod-shaped bacteria on

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10 Great Lab Tricks To Improve Your Performance ...

2020-12-27  In the Lab, Safety First, then Lab Tricks. 8. Working in a lab is not something that you do (or at least you should never do) alone. So you will need to work with other people. There are many tips that can be given related to this, so I’m just

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Tips and Tricks for IC Columns Metrohm Blog

2020-3-30  Tips and Tricks for IC Columns. by Alyson Lanciki Mar 30, 2020 Analysis, Fundamentals, Lab hacks 0 comments. Monitoring and maintaining column performance. One of the basic requirements for ensuring reliable chromatographic analyses is a high-performance separation column

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Solve your problems – KNAUER (U)HPLC Troubleshooting II

2016-9-21  extra column effects: - column overload - cell volume to large - tubing between column and detector to long Solutions check make up of mobile phase adjust flow rate; use higher temperatures check lose fittings - inject smaller volumes - dilute sample - use smaller flow cell - use 0.25 or 0.1 mm ID tubings Troubleshooting: tips and tricks

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Tips, tricks, and troubleshooting for administration

Tips, tricks, and troubleshooting for tasks Interactions between the Commit Date and the Planned Completion Date Projected Duration does not match Planned Duration

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Chips and Tips - Blogs from RSC Publishing

Welcome to Chips Tips – a unique and regularly updated forum for scientists in the miniaturisation field from Lab on a Chip. Chips Tips aims to provide a place where ideas and solutions can be exchanged on common practical problems encountered in the lab

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Tips and Tricks for IC Columns Metrohm Blog

2020-3-30  Tips and Tricks for IC Columns. by Alyson Lanciki Mar 30, 2020 Analysis, Fundamentals, Lab hacks 0 comments. Monitoring and maintaining column performance. One of the basic requirements for ensuring reliable chromatographic analyses is a high-performance separation column. Ion chromatography (IC) users should regularly check the ...

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Practical LC-MS Troubleshooting and Maintenance: A

2017-9-27  MS Troubleshooting Workflow, continued Pass Fail Nominal Mass Calibration off Corrupt Tune Page Lab/Instrument Temperature 6. System Suitability Pass Fail Troubleshoot LC OR Coupling from LC to MS Pass Go to #6 (System Suitability) READY TO RUN! Remember VISUAL INSPECTION is

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GC Troubleshooting: Tips for Quickly ... - Lab Manager

Carefully document all maintenance and troubleshooting activity. Check the obvious and routine things first: look at maintenance logs, check cables and connections, perform a leak check. Isolate system components and steps: confirm proper sample prep, perform a blank run, connect an alternate injector or detector, install a different column.

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Matrigel – Tips, Tricks and Troubleshooting

Matrigel – Tips, Tricks and Troubleshooting. October 17, 2017 • Written by Brandy Sargent . We recently finished our Ask the Expert discussion, “Everything you ever wanted to ask about Corning® Matrigel® Matrix”. During this Ask the Expert session, we had a wide range of questions and discussed everything from storage and handling recommendations to Matrigel for 3D bioprinting.

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GC troubleshooting - GC troubleshooting - Hints and

GC troubleshooting Prevention. Many GC problems can be prevented if the column is properly installed and GC is maintained routinely. For example, replacing septa or liner at regular intervals and keeping the injector and detector clean and well-maintained shoud solve many problems.

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HPLC Troubleshooting Guide: Save Time and

The Thermo Scientific HPLC Troubleshooting Guide app is a free mobile app that offers tips and solutions to a variety of common problems with HPLC analysis. Solutions range from general issues with standard instrument setups to troubleshooting special technologies such as nano LC, fluorescence and charged aerosol detection.

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7 tips for avoiding DNA contamination in your PCR

Tips and troubleshooting. Understanding viral titration—behind the science; ... Use separate sets of pipettes and pipette tips, lab coats, glove boxes, and waste baskets for the pre-PCR and post-PCR areas. ... Stay tuned for more tips and tricks to get the most out of your PCR assays. Back to Blog Front

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COMPLETED - Eliminate the Fear Factor Hands-on

The GC workshop will focus on inlet design, detector design, and troubleshooting, as well as tips, tricks, and tools to develop better methods and analytical capability in the lab. When you check-in for your seminar, you will be assigned a specific rotation through the three workshop topics. Every attendee will participate in all three workshops.

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SPSS Output - Basics and Tips Tricks

Running this syntax opens an output viewer window as shown below. As illustrated, the SPSS output viewer window always has 2 main panes: the output outline is mostly used for navigating through your output items and; the actual output items-mostly tables and charts- are often exported to WORD or Excel for reporting.; In the output outline, you can also delete output items -SPSS often produces ...

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Calculators - Chromatography Calculator HPLC -

Bookmark this page (Ctrl-D) for easy access. Greater Wilmington, NC USA. Contact Us. Phone: +1 732 380 8900 Fax: +1 910 769 9435 Toll-free USA: 1 888 248 4972 Click this link to Email

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Practical LC-MS Troubleshooting and Maintenance: A

2017-9-27  MS Troubleshooting Workflow, continued Pass Fail Nominal Mass Calibration off Corrupt Tune Page Lab/Instrument Temperature 6. System Suitability Pass Fail Troubleshoot LC OR Coupling from LC to MS Pass Go to #6 (System Suitability) READY TO RUN! Remember VISUAL INSPECTION is

More

GC troubleshooting - GC troubleshooting - Hints and

GC troubleshooting Prevention. Many GC problems can be prevented if the column is properly installed and GC is maintained routinely. For example, replacing septa or liner at regular intervals and keeping the injector and detector clean and well-maintained shoud solve many problems.

More

7 tips for avoiding DNA contamination in your PCR

Tips and troubleshooting. Understanding viral titration—behind the science; ... Use separate sets of pipettes and pipette tips, lab coats, glove boxes, and waste baskets for the pre-PCR and post-PCR areas. ... Stay tuned for more tips and tricks to get the most out of your PCR assays. Back to Blog Front

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Troubleshooting RNA Isolation - Bitesize Bio

2014-3-19  As widely used as it is, isolating RNA remains one of the more finicky protocols. Just about anyone who has performed the technique has their own personal tips and tricks to successfully isolate intact RNA from their samples with consistency. Although RNA can be somewhat unpredictable since it is so labile, there are a few common problems that occur that can be solved.

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Tips Tricks: Sensitivity Gains in LC-MS Sigma-Aldrich

2021-3-1  A decrease in column internal diameter (i.d.), while geometrically scaling injection volume and flow rate accordingly, is a simple means of also improving sensitivity of a given separation. A possible and frequent but often overlooked source of contamination in an LC-MS run is the chromatographic column

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Tips and tricks: Recognizing and preventing side

2015-5-12  Tips and tricks: Recognizing and preventing side reactions in Karl Fischer titration. This article explains how side reactions in Karl Fischer titration can be recognized and offers remedies for the different kinds of side reactions that occur depending on the sample that is analyzed.

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COMPLETED - Eliminate the Fear Factor Hands-on

The GC workshop will focus on inlet design, detector design, and troubleshooting, as well as tips, tricks, and tools to develop better methods and analytical capability in the lab. When you check-in for your seminar, you will be assigned a specific rotation through the three workshop topics. Every attendee will participate in all three workshops.

More

SPSS Output - Basics and Tips Tricks

Running this syntax opens an output viewer window as shown below. As illustrated, the SPSS output viewer window always has 2 main panes: the output outline is mostly used for navigating through your output items and; the actual output items-mostly tables and charts- are often exported to WORD or Excel for reporting.; In the output outline, you can also delete output items -SPSS often produces ...

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Controlling Contamination in LC/MS

2016-8-24  CONTROLLING CONTAMINATION IN LC/MS SYSTEMS 715001307 REV.. G 4 OF 33 Use ultra‐pure water Use ultra‐pure water (defined as water that has been purified through a system that targets contaminants detrimental to UPLC®/MS and HPLC/MS systems). Note: See Recommended water purification process, page 29. Ultra‐pure water is sterile and contains no particles greater than

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Calculators - Chromatography Calculator HPLC -

Bookmark this page (Ctrl-D) for easy access. Greater Wilmington, NC USA. Contact Us. Phone: +1 732 380 8900 Fax: +1 910 769 9435 Toll-free USA: 1 888 248 4972 Click this link to Email

More